Identification of Reduced Nicotinamide Adenine Dinucleotide Phosphate-Dependent Aldehyde Reductase in A Rhodotorula Strain
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منابع مشابه
Purification and Characterization of a Reduced Nicotinamide Adenine Dinucleotide Phosphate-linked Aldehyde Reductase from Brain*
The presence of a nonspecific NADPH-linked aldehyde reductase (alcohol: NADP oxidoreductase, EC 1.1.1.2) was observed in various areas of bovine brain in vitro. In the midbrain, hypothalamus, and pons, the rate of NADPH oxidation, with p-nitrobenzaldehyde as substrate, was approximately 4.0 nmoles per min per mg of protein; but somewhat lower values were obtained when other regions of the brain...
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Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
متن کاملThe characterization of two reduced nicotinamide-adenine dinucleotide phosphate-linked aldehyde reductases from pig brain.
1. NADPH-linked aldehyde reductase from pig, ox and rat brain exhibits non-linear reciprocal plots when partially purified enzyme preparations are studied. 2. In pig brain this non-linearity is due to the presence of two distinct aldehyde reductases, which can be separated by DEAE-cellulose chromatography. 3. These two enzymes can be distinguished by several criteria, including pH optima, Micha...
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1. The assimilatory nitrite reductase of the N(2)-fixing bacterium Azotobacter chroococcum was prepared in a soluble form from cells grown aerobically with nitrate as the nitrogen source, and some of its properties have been studied. 2. The enzyme is a FAD-dependent metalloprotein (mol.wt. about 67000), which stoicheiometrically catalyses the direct reduction of nitrite to NH(3) with NADH as th...
متن کاملRegulation of the reduced nicotinamide adenine dinucleotide phosphate-ferredoxin reductase system in Clostridium kluyveri.
The mechanism of regulation of NADPH-ferredoxin reductase was studied in cell-free lysates of Clostridium kluyveri. The following activities, which are assumed to be linked to the enzyme, were investigated: ferredoxin reduction by NADPH, NADPf reduction by reduced ferredoxin, transhydrogenation from NADPH to NAD+, and methyl viologen reduction by NADPH. Ferredoxin reduction by NADPH is controll...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.100.1.110-116.1969